Islamabad, Nov. 14 (TNS): A team of Pakistani scientists has developed a low-cost nanoparticle that can heal burned skin and chronic ulcer wounds, a technology website reported.
According to report published by MIT Technology Review Pakistan, Dr Muhammad Yar and his team at the Interdisciplinary Research Centre in Biomedical Materials (IRCBM) at COMSATS Institute of Information Technology (CIIT) have successfully tested the technique on chicken egg shells and rat skin.
The skin substitute is made of a hydrogel filled with zinc oxide and zinc peroxide nanoparticles to support formation of blood vessels needed to heal burn wounds.
The findings have recently been published in Materials and Design, an internationally-reputed scientific journal.
A World Health Organization (WHO) report published in 2016 had estimated that 265,000 deaths are caused by burns every year – the vast majority of these were reported from lower and middle-income countries.
The only FDA-approved skin for burn patients available in the market is named Integra – a dermal regeneration template. A four centimeter square patch of Integra costs around Rs 80,000.
Most other synthetic skin substitutes available in the market for tissue regeneration are equally expensive but are often unstable under normal storage conditions. Another problem is that these products often fail to support angiogenesis – formation of new blood vessels in the affected part of the skin.
Angiogenesis is crucial in healing of deep wounds as it facilitates growth of natural routes for oxygen and delivery of other nutrients to cells enabling tissue regeneration.
The skin substitute prepared by Dr Yar has the potential to overcome these problems. It supports blood vessels formation and is based on affordable indigenous raw materials. The hydrogel has been prepared by crosslinking two naturally occurring biopolymers.
The research team introduced the hydrogels to an eight-day-old fertilized chicken eggs hatched in an incubator. The egg shells were cut and the hydrogel patch was applied onto the chorioallantoic membrane (CAM) in order to investigate its angiogenic potential. The eggs were then sealed carefully and placed inside the incubator at 37 degrees centigrade and 55 per cent humidity. On 14th day, angiogenesis was quantified by taking light microscope pictures. The results showed that the hydrogels containing zinc peroxide nanoparticles had better angiogenic activity and encouraged formation of new blood vessels.
Later, they grafted the material on a rat with deep wounds and saw positive results.
The hydrogels were prepared from a matrix of biopolymers and nanoparticles. These polymers can hold large amounts of water due to their physical qualities. The team used plant cellulose, which is a non-toxic and inexpensive polymer.
In addition, another biopolymer extracted from marine shells and often discarded as waste in Pakistan was utilized to prepare these hydrogels. Finally, the hydrogels were loaded with zinc oxide and zinc peroxide nanoparticles. The synthetic graft was then made to undergo various chemical and biological tests.
The zinc oxide and zinc peroxide particles used in the graft were a few nanometers in size, making them roughly one billionth part of a meter. Due to this size factor, the nanoparticles provide high surface area and hence, enhanced bioactivity.
Both metal oxides are being widely used in various fields from industrial applications to use in medicines. Zinc oxide is an antibacterial agent. Its nanoparticles have been used in biomaterials to heal wounds and are being studied widely for use in tissue engineering. Zinc peroxide is more easily available making it a potential candidate for preparation of affordable materials needed in tissue engineering applications.
